AATCC 100:2004 pdf download.Antibacterial Finishes on Textile Materials: Assessment of
1. Purpose and Scope 1.1 This test method provides a quanti- tative procedure for the evaluation of the degree of antibacterial activity. Assess- ment of antibacterial finishes on textile materials is determined by the degree of antibacterial activity intended in the use of such materials. If only bacteriostatic activity (inhibition of multiplication) is intended, a qualitative procedure which clearly demonstrates antibacterial activity as contrasted with lack of such activity by an untreated specimen may be accept- able. However, if bactericidal activity is intended or implied, quantitative evalua- tion is necessary. Quantitative evaluation also provides a clearer picture for possi- ble uses of such treated textile materials.
2. Principle
2.1 Swatches of test and control textile materials are tested qualitatively for anti- bacterial activity by AATCC Method 147. Those showing activity are evaluated quantitatively. Test and control swatches are inoculated with the test organisms. After incubation, the bacteria are eluted from the swatches by shaking in known amounts of neutralizing solution. The number of bacteria present in this liquid is determined, and the percentage reduction by the treated specimen is calculated.
4.1 Both the qualitative and quantita- tive tests should be carried out by persons with training and experience in the use of bacteriological techniques. The U.S. De- partment of Health and Human Services publication, Biosafety in Microbiological and Biomedical Laboratories , should be : consulted (see 13.1).
4.2 CAUTION: Some of the bacteria used in this test are capable of infecting humans and producing disease. There- fore, every necessary and reasonable pre- caution must be taken to eliminate this risk to the laboratory personnel and to personnel in the associated environment. Wear protective clothing and respiratory protection that prevents penetration by the bacteria.
4.3 Good laboratory practices should be followed. Wear safety glasses in all laboratory areas.
4.4 All chemicals should be handled with care.
4.5 An eyewash/safety shower should be located nearby for emergency use.
4.6 Sterilize all contaminated samples and test materials prior to disposal.
4.7 Exposure to chemicals used in this procedure must be controlled at or below levels set by govermment authorities (e.g., Occupational Safety and Health Adminis- tration's [OSHA] permissible exposure limits [PEL] as found in 29 CFR . 1910. 1000 of January 1, 1989). In addition, the American Conference of Governmen- tal Industrial Hygienists (ACGIH) Thresh- old Limit Values (TLVs) comprised of time weighted averages (TLV-TM), short term exposure limits (TLV-STEL) and ceiling limits (TLV-C) are recommended as a gen- eral guide for air contaminant exposure which should be met (see 13.2).AATCC 100 pdf download.