AATCC 147:1998 pdf download.Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method
1.1 The objective is 10 detect bacterio- static activity on lextile materials. The re- suts of using this procedure have been demonstrated by Comnmite RA3I to be rproducible by various laboratories working with materials containing resid- ual Bmounts of antibacterial agents (as determined by chemical assay) after mul- liple standard washings. The method is aseful for obtaining a rough estimate of activity in that the growth of the inocu- lum oganism decreases from one end of each streak to the other and from one streak to the next resulting in increasing degres of sensitivity. The size of the ZzopC of inhibition and the narrowing of thesteaks caused by the presence of the abibactcrial agent perpit an estimate of tbe residual antibacterial activity after utiple washings.
2.1 Specimens of the test material, in-cluding corresponding untreaied controls of the same material, are placed in inti- mate contact with nutrient agar (see 7.1 and 7.4) which has been previously streaked with an inoculum of a test bacte- rium. After incubation, a clear area of in. terrupted growth undemeath and along the sides of the test material indicates an- tibacterial activity of the specimen. A standard strain of bacteria is used which is specific to the requirements of the ma- terial under test. If no other bacterial spe- cies is specified, Saphylococcus aureus may be used as a representative Gram positive organism. Other recommended strains are listed below in Section 6.
4. Safety Precautions NOTE: These safety precautions are for information purposes only. The pre- cautions are ancillary to the testing proce- dures and are not intended to be all inclu- sive. It is the user's responsibility to use safe and proper techniques in handling materials in this test method. Manufac- turers MUST be consulted for specific details such as material safety data sheets and other manufacturer's recommenda. tions. Al OSHA standards and rules must also be consuted and followed.
4.1 This test should be performed only by trained personnel. The U.S. Depart- ment of Health and Human services pub- lication Biosafery in Microbiological and Biomedical Laboratories should be con- sulted (sce 13.1).AATCC 147 pdf download.